Fig. 1.

A proteomics screen to identify proteins that are recruited to sites of DNA damage. (A) Overview of the proteomics strategy used to identify proteins that are preferentially recruited to sites of DNA damage. Arg, arginine; Lys, lysine. (B) UV induced monoubiquitination of FANCD2 as well as phosphorylation of Chk1 and H2AX. (C) Untreated HeLa cells were mixed with cells exposed to UV light and fractionated. The effectiveness of separation of soluble, chromatin-bound, and nuclear matrix-associated proteins was determined by Western blotting. (D) Proteins in the chromatin fraction with a median vista ratio >1 are plotted in the order of increasing ratio. A ratio >1 suggests enrichment in the chromatin fraction after DNA damage.