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. 2010 Oct 11;107(43):18475–18480. doi: 10.1073/pnas.1012946107

Fig. 3.

Fig. 3.

NuRD components CHD4 and MTA1 accumulate at DNA lesions following UV laser microirradiation. (A) U2OS cells expressing MTA1-GFP pretreated for 1 h with either DMSO (control) or 1 μM PARP inhibitor KU-0058948 were microirradiated with a UV laser, fixed after 10 min, and immunostained with anti-GFP and anti-γH2AX antibodies. (B) U2OS cells pretreated for 1 h with DMSO or 2 μM PARP inhibitor KU-0058948 were microirradiated with a UV laser, fixed after 10 min, and immunostained with anti-CHD4 and anti-γH2AX antibodies. (C) Quantification of the data shown in A. (D) U2OS cells expressing GFP were transfected with the indicated siRNAs, mixed with cells expressing dsRed, mock-treated or exposed to various doses of IR, and analyzed by FACS after 9 d in a competition assay.