Fig. 2.

Obstruction-induced luminal stretch increases cilia beat frequency. (A) Pronephric obstruction at 48 hpf induced an increased cilia beat rate that closely followed tubule luminal stretch with a latency of 15–30 min. (A, Inset) After stabilization of lumen diameter at 0, 30, 90, and 210 min, cilia beat rate was directly proportional to lumen diameter. Error bars represent SEM. (B) Representative images of GFP-positive E11-9 transgenic tubule segment showing distension at time points indicated.