Fig. 3.

DNAM-1 is involved in donor CD8⁺ T-cell proliferation in recipient mice after transplantation. (A) After sublethal irradiation, B6C3F1 mice received splenocytes from DNAM-1 WT or KO B6 mice. The infiltrating cells in the liver and small intestine in recipient mice (n = 3) on day 14 after transplantation were separated, and each donor-derived (H-2K^k−) lymphocyte subset was determined by flow cytometry. Data are representative of two independent experiments. (B) Resting CD8⁺ T cells purified from naive DNAM-1 WT or KO B6 mice (Left) and donor effector CD8⁺ T cells purified from B6C3F1 mice that received WT or KO B6 splenocytes (Right) were labeled with CFSE, cocultured with mitomycin C-treated syngeneic (B6) or allogeneic (B6C3F1) splenocytes for 3 d, and analyzed by flow cytometry. Data are representative from three independent experiments with similar results. *P < 0.05. Error bars show SD.