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. 2001 Mar 1;29(5):1054–1060. doi: 10.1093/nar/29.5.1054

Figure 2.

Figure 2

Binding of Cbf1p and Cbf1ΔN209p to CEN DNA. (A) Cbf1p binding. Lanes 1–15, increasing Cbf1p concentrations: lane 1, 0.63 nM; lane 2, 0.89 nM; lane 3, 1.04 nM; lane 4, 1.25 nM; lane 5, 1.39 nM; lane 6, 1.56 nM; lane 8, 1.79 nM; lane 9, 2.08 nM; lane 10, 2.50 nM; lane 11, 3.13 nM; lane 12, 4.17 nM; lane 13, 6.25 nM; lane 14, 12.5 nM; lane 15, 25 nM. (B) Cbf1ΔN209p binding. Lanes 1–15, increasing Cbf1ΔN209p concentrations: lane 1, 1.41 nM; lane 2, 2.01 nM; lane 3, 2.35 nM; lane 4, 2.82 nM; lane 5, 3.13 nM; lane 6, 3.52 nM; lane 8, 4.02 nM; lane 9, 4.69 nM; lane 10, 5.63 nM; lane 11, 7.05 nM; lane 12, 9.37 nM; lane 13, 14.07 nM; lane 14, 28.13 nM; lane 15, 56.25 nM. M, marker DNA in steps of 1 kb; F, free CEN DNA; C, complexed CEN DNA.