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. 2001 Mar 1;29(5):1054–1060. doi: 10.1093/nar/29.5.1054

Figure 5.

Figure 5

p64-induced stimulation of Cbf1p DNA binding. (A) Coomassie stained SDS–gel of purified, recombinant CBF3 subunit p64 (lane 1). M, molecular weight standard proteins. (B) CEN 9 DNA was incubated with 10, 20 and 40 nM p64, and reaction mixtures analysed by gel shift (lanes 1, 2 and 3, respectively). No complex is observed. (C) CEN 9 DNA was incubated with 0.2 nM of Cbf1p (lanes 1 and 2) or Cbf1ΔN209p (lanes 3 and 4) in the absence (lanes 1 and 3) or presence (lane 2 and 4) of 20 nM p64.