Table 1. 1H T1 (s ± 0.02 s) relaxation times in the PEG6 tethered decamer measured in Na2HPO4 (sample 4) and Na2HPO4 + K3PO4 buffer (sample 5, in parentheses) in H2O at pH 6.0, 30°Ca.
|
5′-G1 |
C2 |
G3 |
T4 |
T5 |
G6 |
T7 |
C8 |
G9 |
C10-3′ |
H6/H8 | 0.32(0.57) | 0.45(0.65) | 0.30(0.42) | 0.54(0.77) | 0.42(0.65) | 0.48(0.68) | 0.62(0.78) | 0.40(0.63) | 0.31(0.80) | 0.63(0.80) |
H1′ | 0.68(0.92) | 0.54(0.84) | 0.48(0.74) | 0.47(0.76) | 0.67(0.85) | |||||
CH3 | 0.38(0.68) | 0.32(0.60) | 0.41(0.68) | |||||||
H5 | (0.56) | (0.82) | 0.62(0.86) | |||||||
NH | 0.41(0.53) | 0.45(0.66) | 0.47(0.57) | |||||||
NH | 0.45(0.53) | 0.51(0.53) | 0.41(0.53) | |||||||
H5 | 0.63(0.86) | 0.41(0.70) | 0.36(0.61) | (0.56) | ||||||
H2 | 0.50(0.80) | 0.42(0.72) | 0.33(0.63) | |||||||
H1′ | 0.64(0.85) | (0.77) | (0.76) | (0.70) | 0.68(0.92) | |||||
H6/H8 |
0.61(0.78) |
0.35(0.60) |
0.42(0.63) |
0.38(0.63) |
0.31(0.54) |
0.42(0.63) |
0.38(0.63) |
0.32(0.49) |
(0.65) |
0.32(0.57) |
3′-C20 | G19 | C18 | A17 | A16 | C15 | A14 | G13 | C12 | G11-5′ |
aPEG linker resonances do not show differences in shape in both solutions and T1 relaxation times are very close and on average equal 0.58 and 0.62 s, respectively.