Skip to main content
. 2001 Mar 1;29(5):1027–1033. doi: 10.1093/nar/29.5.1027

Figure 1.

Figure 1

Figure 1

PDGF-B mRNA expression is induced in WKY12-22 cells exposed to nogalamycin. WKY12-22 cells were exposed to 10 µM nogalamycin for various times, and total RNA was prepared using TRIzol reagent according to the manufacturer’s instructions. The RNA was reverse-transcribed and the cDNA was used as the template for PCR. (A) Time-course of nogalamycin-inducible PDGF-B expression. (B) Actinomycin D pretreatment (10 µg/ml for 2 h) blocks PDGF-B expression inducible by nogalamycin. Cycle-based PDGF-B PCR (bottom panel) was performed on 8 h samples without actinomycin D preincubation. The data are representative of two independent determinations.