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Nogalamycin stimulates PKC-ζ (Thr410) phosphorylation in WKY12-22 cells. Cells were exposed to 10 µM of nogalamycin for various times prior to resolution of the cytoplasmic extracts by electrophoresis and western blot analysis using antibodies to the Thr410 phosphorylated form of PKC-ζ. The data are representative of two independent determinations.
