Figure 2.

Nogalamycin increases PDGF-B promoter-dependent gene transcription. WKY12-22 cells were transiently transfected with 8 µg of PDGF-B promoter–CAT construct d26 or d75 and CAT activity in the cell lysates was determined after 48 h. CAT activity was normalised to the concentration of protein in the lysates. Nogalamycin was added (2 and 10 µM final concentration) to the transfectants 24 h prior to harvesting. The data are representative of two independent determinations.