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The zinc finger binding site in the proximal PDGF-B promoter mediates nogalamycin-inducible PDGF-B promoter-dependent gene expression. WKY12-22 cells were transiently transfected with construct d26, d77 or md77. After 24 h, the transfectants were incubated with 10 µM of nogalamycin for a further 24 h prior to harvest. The cells were lysed and CAT activity was normalised to the concentration of protein. The data are representative of two independent determinations.
