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PDGF-B transcription inducible by nogalamycin is Sp1-dependent. WKY12-22 cells were cotransfected with 8 µg of d26 and 5 µg of either pEBG or pEBG-DN Sp1. After 24 h, 10 µM nogalamycin was added to transfectants. CAT activity in the lysates was determined 48 h after transfection and normalised to the concentration of protein. The data are representative of two independent determinations.
