Figure 6.

Nogalamycin increases Sp1 DNA binding to the proximal PDGF-B promoter. (A) EMSA was performed using nuclear extracts of WKY12-22 cells exposed to 10 µM nogalamycin for the indicated times and ³²P-labelled Oligo B. Binding reactions and non-denaturing gel electrophoresis were performed as described in Materials and Methods. (B) Nogalamycin increases Sp1 DNA binding in a dose-dependent manner. Nuclear extracts from WKY12-22 cells exposed to various concentrations of nogalamycin for 5 h were incubated with ³²P-labelled Oligo B (5′-GCT GTC TCC ACC CAC CTC TCG CAC TCT-3′). Binding reactions and non-denaturing gel electrophoresis were performed as described in Materials and Methods. Nucleoprotein complexes were visualised by autoradiography. The data are representative of two independent determinations.