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Nogalamycin phosphorylates endogenous Sp1 but not Egr-1 or Sp3. Nuclear extracts from WKY12-22 cells exposed to 10 µM nogalamycin for various times were resolved by electrophoresis prior to transfer to PVDF membranes and exposure to polyclonal antibodies to Sp1, Sp3 or Egr-1. Where indicated, the 8 h sample was incubated with CIP prior to western blot analysis for Sp1. The data are representative of two independent determinations.
