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Nogalamycin activation of the PDGF-B promoter is PKC-ζ-dependent. WKY12-22 cells were cotransfected with 8 µg of d26 and 5 µg of either CMV-Flag or pPKC-ζ-kw. Nogalamycin (10 µM) was added to the transfectants 24 h post-transfection prior to harvest 24 h later. CAT activity was normalised to the concentration of protein in the extracts. The data are representative of two independent determinations.
