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DN PKC-ζ ablates Sp1 phosphorylation in WKY12-22 cells. Cells were transfected with 30 µg of CMV-Flag or pPKC-ζ-kw 24 h prior to the addition of nogalamycin (2 µM). Nuclear extracts were prepared 24 h later. Nuclear extracts were resolved by electrophoresis prior to transfer to PVDF membranes and exposure to polyclonal Sp1 antibodies. The data are representative of two independent determinations.
