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. 2010 Oct 25;432(Pt 1):9–19. doi: 10.1042/BJ20100308

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© 2010 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(A) Paraffin-embedded sections were prepared from paraformaldehyde-fixed tissues and stained with anti-phopho-Smad2/3 antibody linked to Alexa Fluor® 488 (green); the nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole; blue). Sections were imaged using Simple PCI software. (B) Images were quantified for phospho-Smad2/3 localization in the nucleus using Simple PCI software. Mean green intensities (n=6, per group) were calculated, analysed by ANOVA and the Newman–Keuls test and expressed (± S.E.M). #P≤0.05.