Figure 2.

Characterization of GH4 nuclear factors binding to the EBS-RRE probe. GH4NE (1.5 µg) was incubated with a radiolabeled oligonucleotide corresponding to EBS of the RRE (–220 to –205) of the rPRL promoter. Protein–DNA complexes were competed with 400-fold molar excess of cold, unlabeled oligonucleotides as described in Figure 1. Specific protein–DNA complexes are indicated by ‘A’ and non-specific complexes are denoted with ‘NS’. ‘Free’ indicates free probe.