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Gel shift analysis of GH4 and HeLa nuclear extracts with the BTE and EBS-RRE probes. GH4 or HeLa nuclear extract (1.5 µg) was incubated with the BTE or EBS-RRE probes (as indicated) as described above. ‘A’ indicates a specific protein–DNA complex, and ‘C’ indicates a HeLa-specific protein–DNA. Non-specific complexes are denoted by ‘NS’ and ‘B’. ‘Free’ indicates free probe.
