Figure 7.

UV-crosslinking of GH3 nuclear factors that bind to the BTE and EBS-RRE probes. (A) The peak 1 heparin–Sepharose fraction (2 µl) and input (1 µl) were incubated with the BTE probe, containing two bromodeoxyuridine substitutions, in a standard EMSA reaction, as described in the Materials and Methods. The reaction was incubated for 20 min at room temperature, exposed to UV light (254 nm) for 30 min at 4°C and analyzed by SDS–PAGE. (B) The peak 1 heparin–Sepharose fraction (2 µl) and input (1 µl) were incubated with the EBS-RRE probe in a standard EMSA reaction, as described in (A). Molecular weight markers are indicated to the left.