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. Author manuscript; available in PMC: 2010 Nov 4.
Published in final edited form as: Plant J. 2008 Sep 19;56(5):704–715. doi: 10.1111/j.1365-313X.2008.03631.x

Figure 4. ORTH1 proteins with either RING domain, but not the PHD, have E3 ligase activity.

Figure 4

Truncations of ORTH1 (name and schematic form under their respective western blots) were expressed as GST fusions and tested for in vitro ubiquitylation activity.

(a-e) Top, anti-ubiquitin immunoblot to detect ubiquitylated products; bottom, anti-GST to visualize E3 ligase. Reactions lacking either E1, E2 or E3 (-E1, -E2, -E3, respectively) are compared to comparable reaction with all components (T).

(e) mPHD has the CCHC of the core metal ligands, positions 3-6, all changed to alanine. Full length ORTH1 assays (a) were included for comparison.