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Expression of RB69 and T4 RegA proteins from pAS1 vectors. Total cell extracts from 5 µl of uninduced cell culture (lane 3), 5 µl of nalidixic acid-induced cell cultures expressing T4 RegA protein (lane 4), 10 µl of induced cell cultures expressing RB69 RegA protein from plasmids pG62A (lane 5) (see Fig. 1), pCIIB (lane 6) and WT pAS1-RB69 regA (lane 7), respectively, were applied to a 15% SDS–polyacrylamide gel. Lane 1, molecular weight markers; lane 2, purified T4 RegA protein.
