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. 2001 Mar 1;29(5):1175–1184. doi: 10.1093/nar/29.5.1175

Figure 4.

Figure 4

Gel mobility shift assay of RB69 RegA protein binding to RE RNAs. 32P-5′-end-labeled 18mer RB69 gene 44 RE RNA (5′-AUGAGGAAAAUUACAUGA-3′) (lanes 1–4), 16mer T4 gene 44 RE RNA (5′-AAUGAGGAAAUUAUG-3′) (lanes 5–7) and 16mer T4 gene 44 G–9U RE RNA (5′-AAUUAGGAAAUUAUG-3′) (lanes 8–10) were incubated with cell supernatants containing no RegA protein (lane 2) or 20 nM RB69 RegA protein (lanes 3, 4, 6, 7, 9 and 10). Samples were analyzed by electrophoresis on a native 6% polyacrylamide gel. Gels were dried and then analyzed on a PhosphorImager (see Materials and Methods).