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. Author manuscript; available in PMC: 2010 Nov 4.
Published in final edited form as: Mol Microbiol. 2009 Aug 4;74(1):29–43. doi: 10.1111/j.1365-2958.2009.06831.x

Figure 6. Effects of mutations in GlpR operators on promoter activity and the appearance of Glp+ mutants.

Figure 6

The graphs show the effects of mutations in GlpR operators, O1 and O4 (see Figure 1A), on (A) PglpFK activity in crp cells grown in LB liquid medium, and (B) Glp+ mutation frequencies in crp cells grown on LB solid medium. The bars represent standard deviation values for three independently conducted experiments. wt: control with no mutation in either O1 or O4; O1: only operator 1 was mutated (see Methods); O4: only operator 4 was mutated; O1O4: both operators were mutated.