Table 1. DNA primers and RNA and DNA templates.

Primers

5′-CCTCTCGTAAAATATG-3′

5′-CCTCTCGTAAAATATGGC-3′

5′-CCTCTCGTAAAATATGGCC-3′

5′-CCTCTCGTAAAATATGGCCTCTTT-3′

Templatesa

RNA 5 10 15 * ** * 30 35

3′-GGAGAGCAUUUUAUACCGGXGAAAUAAGAGGAAAU-5′

DNA 5 10 15 * ** * 30 35

3′-GGAGAGCATTTTATACCGGAGAAATAAGAGGAAAT-5′

^aThe templates are derived from a hypervariable region of the extra-cellular region of the envelope gene of the WMJ-1 strain of HIV-1. Template positions that accept incoming bases in primer extension assays are in bold and denoted by an asterisk. In the RNA template, the X denotes adenosine or hypoxanthine.