Table 2. HIV-RT steady-state kinetic data for RNA templates.

Template basea	dNTP	KM (µM)	Vmax (pM/s)	kcat (s–1)	Second order rate (s–1M–1)	finsb
H20	A	14	60	4.8 × 10–4	3.4 × 101	5.0 × 10–3
H20	C	0.024	206	2.1 × 10–3	8.7 × 104	1.3 × 101
H20	G	83.000	150	1.2 × 10–3	1.4 × 101	2.1 × 10–3
H20	T	0.590	88	7.0 × 10–4	1.2 × 103	1.8 × 10–1
A20	H	530.000	110	8.8 × 10–4	1.7 × 100	2.4 × 10–4
C17	H	0.043	104	8.3 × 10–4	1.9 × 104	8.0 × 100
G19	H	92.000	76	6.1 × 10–4	6.6 × 100	8.3 × 10–4
U25	H	125.000	44	3.5 × 10–4	2.8 × 100	5.9 × 10–4
A20	A	97.000	51	4.1 x 10–4	4.2 x 10–6	6.2 × 10–4
A20	C	6.200	32	2.6 × 10–4	4.1 × 101	6.1 × 10–3
A20	G	120.000	16	1.3 × 10–4	1.1 × 100	1.6 × 10–4
C17	A	41.000	60	4.8 × 10–4	1.2 × 101	4.9 × 10–3
G19	A	78.000	18	1.4 × 10–4	1.8 × 100	2.3 × 10–4
C17	G	0.099	30	2.4 × 10–4	2.4 × 103
G19	C	0.039	39	3.1 × 10–4	8.0 × 103
A20	T	0.052	44	3.5 × 10–4	6.8 × 103
U25	A	0.140	84	6.7 × 10–4	4.8 × 103

^aNumbers correspond to template base positions from Table 1.

^bFrequency of insertion (f_ins) is defined in the legend to Figure 1.