Figure 5. Discrete roles of individual factors on deletion size during Distal-EJ.

ATM and Xrcc4 suppress longer deletions, whereas Nbs1 and DNA-PKcs promote short deletions. Distal-EJ products were amplified and cloned for sequencing analysis from GFP+ sorted samples following co-expression of I-SceI and Trex2. Shown is a summary of sequence analysis for WT cells, WT cells treated with ATMi, ATM^−/− cells, Nbs1^n/h cells, Nbs1^n/h cells treated with ATMi, DNA-PKcs^−/− cells, and Xrcc4^−/− cells. Shown are the numbers of products (out of 30 total, sequences in Table S1) in five different classifications: +1 insertion (ins.), 1 to 5 nt. deletions (del.), 6 to 9 nt. del., 10 to 19 nt. del., and ≥20 nt. del.