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. 2010 Nov 4;6(11):e1001178. doi: 10.1371/journal.ppat.1001178

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Slater et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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HBECs were transfected with siRNA specific for RIG-I, MDA5 or control siRNA, infected with RV1B or RV16 and vRNA determined by PCR at 24 h (A) or RV release by titration at 48 h (B). BEAS-2B cells were transfected with 0.5 µg RIG-IC or pEF-BOS control, and RV16 vRNA (C) and virus release (D) determined. mRNA data was generated from 5 independent experiments utilizing 3 independent HBEC donors, 2 experiments per donors 1,2 and a one experiment for donor 3; and 4 independent experiments for BEAS-2B cells.