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. 2010 Nov 4;6(11):e1001178. doi: 10.1371/journal.ppat.1001178

Figure 9. Expression of RIG-I and MDA5 mRNA and protein in wildtype and IFNAR1 deficient mice.

Figure 9

Wildtype 129/SvJ and IFNAR1−/− mice were infected with RV1B and lung protein and mRNA analysed over time. In wildtype mice, and to a lesser extent, IFNAR1−/− mice, RV1B induced IFN-β mRNA (A), and IFN-λ mRNA only in wildtype mice (B), and induced RIG-I in both wildtype and IFNAR1−/− mice (C). RV1B also induced MDA5 in both wildtype and IFNAR1−/− mice (D). In both wildtype and IFNAR1−/− mice, RIG-I and MDA5 mRNA induction was evident by 8 h post infection. RV1B also induced RIG-I and MDA5 protein by 8 h in both wildtype and IFNAR1−/− mice, measured by western blot (E), and RIG-I and MDA5 protein as a ratio of α-tubulin protein measured by densitometry over time is also shown (F&G respectively) *p<0.05, **p<0.01 ***p<0.001 versus time 0 h or as indicated.