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. 2010 Nov 4;6(11):e1001200. doi: 10.1371/journal.pgen.1001200

Table 1. Summary of all UNC-104 alleles and transgenes and their behaviour in multiple assays.

Allele Transgene In vitro PIP2 binding In vivo PIP2 sensitivity In vivo motor on synaptic vesicles Rescue of unc-104 function UNC-104 Protein levels and localization
Wild type High Insensitive (saturated?) High ---- High, largely in synapse-rich regions
unc-104(e1265) encodes UNC-104(D1497N) Poor insensitive Very low ---- Very low, residual in synapse-rich regions
unc-104(e1265tb107) encodes UNC-104(D1497N R1501Q) Medium sensitive More than in unc-104(e1265) ---- Medium, residual in synapse-rich
unc-104(e1265tb120) encodes UNC-104(D1497N M1540I) Medium sensitive More than in unc-104(e1265) ---- Medium, residual in synapse-rich
UNC-104::GFP High ------ ------ Full Similar to wild type
UNC-104(D1497N)::GFP Poor ------ ------ No No GFP tagged protein observed
UNC-104(D1497N)::GFP very high copy number ------ ------ ------ No Similar to wild type
UNC-104(M1540I)::GFP ------ ------ ------ Full Similar to wild type
UNC-104(D1497N M1540I)::GFP Medium ------ ------ Nearly full Similar to wild type
UNC-104(D1497N R1501Q)::GFP Medium ------ ------ Nearly full Similar to wild type
UNC-104(R1501Q)::GFP ------ ------ ------ Full Similar to wild type
UNC-104(W1549A)::GFP ------ ------ ------ Full Similar to wild type
UNC-104(D1497N M1540I W1549A)::GFP Poor ------ ------ Poor No GFP tagged protein observed
UNC-104(KK1463/4AA)::GFP high expression Poor [12] sensitive ------- Poorer than UNC-104(D1497N M1540I)::GFP Similar to wild type
UNC-104(R1496A)::GFP high expression Poor [12] sensitive ------- Poorer than UNC-104(D1497N M1540I)::GFP Similar to wild type