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. 2010 Oct 18;107(44):19073–19078. doi: 10.1073/pnas.1009803107

Fig. 3.

Fig. 3.

Requirements for the induction of post-LTP at thalamo-amygdala synapses. (A) LTP in thalamic input was induced by presynaptic stimulation for 2 min with paired pulses (50-ms interpulse interval). Unlike pre-LTP, the recorded LA neuron here was voltage-clamped at +30 mV during the induction. (B) Post-LTP, observed under control conditions (n = 6), was blocked by 20 mM BAPTA in the recording pipette solution (n = 10). Postsynaptic depolarization to +30 mV without presynaptic stimulation did not result in LTP (n = 5). (Insets) Averaged EPSCs recorded before and 35 min after the time point when LTP-inducing stimulation was delivered. (C) Post-LTP in thalamic input in the presence of UBP296 (1 μM; n = 4), D-AP5 (50 μM; n = 5), or D-AP5 + nitrendipine (nitrendipine, 10 μM; n = 5). (Insets) Averaged EPSCs recorded before and 35 min after the delivery of LTP-inducing stimulation. (D) Summary of LTP experiments at thalamo-amygdala synapses (control, n = 6; BAPTA, n = 10, P < 0.01 vs. control LTP; depolarization alone, n = 5, P < 0.01 vs. control LTP; UBP296, n = 4, P = 0.51 vs. control; D-AP5, n = 5, P = 0.01 vs. control; D-AP5 + nitrendipine, n = 5, P < 0.01). Error bars indicate SEM.