Table 2.

The fitness of the sHLA peptides sequences to the HLA consensus binding motifs of the donors

Subject	HLA allele	% high-certainty peptides	HLA-A+B supertype
Healthy 1; 250 high-certainty peptides	HLA-A01	37	A01
	HLA-A02	12	A02
	HLA-B35HLA-Cw04	49	B07*
	Unassigned motif	2
Healthy 6; 180 high-certainty peptides	HLA-A02	15	A02
	HLA-A68	48	A03
	HLA-B18	24	B44
	HLA-B55HLA-Cw07	7	B07
	HLA-Cw03	4	*
	Unassigned motif	2
Healthy 4; 200 high-certainty peptides	HLA-A24HLA-Cw07	42	A24
	HLA-A68	30	A03
	HLA-B49	12	B44
	HLA-Cw01	7	*
	Unassigned motif (HLA-B22)	12
AML-1; 330 high-certainty peptides	HLA-A03	33	A03
	HLA-B27	6	B27
	HLA-B35HLA-Cw04	48	B07*
	Unassigned motif (HLA-Cw1502)	13
Healthy 5; 244 high-certainty peptides	HLA-A26	17	A01
	HLA-A66	14	A03
	HLA-B38	4	B27
	HLA-B51	44	B07
	Unassigned motif (HLA-Cw12, HLA-Cw14)	21
MM-1; 250 high-certainty peptides	HLA-A02	12	A02
	HLA-A11	33	A03
	HLA-B51	20	B07
	HLA-B52	5	B62
	Unassigned motif (HLA-Cw02, HLA-Cw12)	30
MM-2; 158 high-certainty peptides	HLA-A11	34	A03
	HLA-A26	13	A01
	HLA-B07	8	B07
	HLA-B38	1	B27
	Unassigned motif (HLA-Cw12, HLA-Cw15)	43

Only sHLA peptidomes with above 150 high-certainty identified peptides were selected for this analysis. Fitness to the consensus binding motifs was assigned according to the HLA A and HLA B supertypes (22) and to HLA Cw (*) according to (44).