Fig. 3.

Evolutionary conservation of the inserted glutamine residue. (A) Genomic structure and partial transcript map of AG and FAR. The DNA sequence surrounding the triplet that encodes the additional glutamine (CAG) is magnified in FAR, and the corresponding position is also shown in AG. The two CAG triplets that represent the splice acceptor consensus sequences are underlined in black. In FAR, the CAG duplication slides the acceptor forward, thereby introducing the critical Q173 residue. (B) Phylogenetic tree of species with known FAR orthologs. Those species lacking Q173 are shown in blue, and those known to carry Q173 are shown in red. The presence of Q173 is confined to, but not present in all Lamiids. Among the Solanales, Solanum Lycopersicon, Petunia, and Nicotiana tabacum do not have the additional glutamine. Dotted lines represent the part of the tree that remains uncertain (34). (C) Ectopic expression of the Ipomoea PLE and FAR orthologs, PN and DP, in Arabidopsis. The organ conversions of 35S::PN are identical to those present in 35S::AG, whereas 35S::DP is identical to 35S::FAR.