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. 2010 Oct 13;107(44):18997–19001. doi: 10.1073/pnas.1004380107

Fig. 1.

Fig. 1.

AnkG interferes with apoptosis and binds the host protein p32. (A) CHO cells ectopically expressing GFP or the indicated GFP-tagged C. burnetii Anks were incubated with staurosporine. The nuclei were stained with DAPI, and nuclei of GFP-expressing cells were scored for fragmentation. One hundred nuclei from GFP-expressing cells per sample from four independent experiments were counted. *P < 0.001. (B) HEK 293 cells were cotransfected with plasmids encoding HA-tagged p32 and the indicated GFP-tagged C. burnetii ankyrin repeat-containing proteins. Proteins were precipitated from the cell lysates with an anti-GFP antibody and analyzed by immunoblot. The α-GFP blot shows Ank protein levels in the immunoprecipitate (IP), and the α-HA blot shows p32 levels in the immunoprecipitate (IP) and lysate. (C) HEK 293 cells were transfected with a plasmid encoding GFP-AnkG or the GFP control. Proteins were precipitated from the cell lysates with an anti-GFP antibody, and endogenous p32 was analyzed by immunoblotting with an anti-p32 antibody.