Skip to main content
. 2010 Oct 13;107(44):18997–19001. doi: 10.1073/pnas.1004380107

Fig. 3.

Fig. 3.

Translocation of AnkG prevents rapid pathogen-induced apoptosis, allowing L. pneumophila replication in DCs. (A) CHO-FcR cells infected with L. pneumophila harboring the vector pJV400, pJV400-AnkB (AnkB), or pJV400-AnkG (AnkG) were incubated with staurosporine. L. pneumophila was stained with a specific anti-Legionella antibody, and the nuclei were scored by the TUNEL assay. The nuclei of at least 100 infected cells per sample from three independent experiments were counted. *P < 0.01. (B) Bone marrow DCs derived from B6 mice were infected for 6 h with L. pneumophila ΔflaA expressing the pJV400 vector, AnkG, or AnkB or with L. pneumophila ΔdotA expressing AnkG. L. pneumophila was stained with a specific anti-Legionella antibody, and the nuclei of infected cells were scored by the TUNEL assay. Data shown are the mean ± SD of 300 cells counted per each coverslip in triplicate and are representative of two independent experiments. **P < 0.01. (C and D) Representative fluorescence micrographs of B6 DCs infected with (C) L. pneumophila ΔflaA + pJV400, ΔflaA + AnkB, or ΔflaA + AnkG and (D) L. pneumophila ΔflaA expressing AnkG1–69 or AnkG70–339 or L. pneumophila ΔdotA expressing full-length AnkG. DCs were fixed at 10 h postinfection and were stained with an antibody specific for MHC class II (red), DAPI (blue), and an anti-Legionella antibody (green).