Fig. 5.

Cav-1 regulates CS-induced autophagy and apoptosis in vivo. WT C57BL/6 or Cav-1^−/− mice were exposed to chronic CS exposure for 3 mo (each group, n = 5). (A) EM images were scored for number of AVs. The data are represented as AVs per 100 μm²; n = 20 images representative of each group. Western blot analysis and corresponding quantification for the LC3B-II/LC3B-I ratio (B) and cleaved caspase-9 (C) in CS-exposed mouse lungs. (D) MLI scoring of mouse lungs exposed to CS. Data represent mean ± SD; *P < 0.05. Representative images of mouse lungs with H&E staining are shown in Fig. S5.