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. 2010 Oct 18;107(44):18979–18984. doi: 10.1073/pnas.1013387107

Fig. 1.

Fig. 1.

mAb 12D1 reacts with the LAH of HA2. (A) Hemagglutinin monomer. Amino acids 76–130 of HA2 are highlighted in red. (B) Lysates from 293T cells were transfected with pCAGGs-GFP or pCAGGs-GFP HA2 76–130, incubated with mAb 12D1, and pulled down with protein G beads. Pulled-down fractions were blotted with mAb 12D1 or rabbit anti-GFP. Arrows indicate location of the amino acid 76–130 region–GFP fusion protein. Anti-mouse HRP used to detect 12D1-binding reacts with the mouse Ig heavy and light chains within the pulled-down fraction. (C) Structural integrity of the 12D1 epitope within the LAH-KLH conjugate was confirmed by direct-binding ELISA. Hemagglutinin-specific mAb 36A7 does not bind within the amino acid 76–130 region of HA2.