Skip to main content
. 2010 Oct 18;107(44):18944–18949. doi: 10.1073/pnas.1007558107

Fig. 4.

Fig. 4.

SHEP1 constitutively associates with CasL, and SHEP1 promotes CasL hyperphosphorylation. (A) Immunoprecipitated CasL from BAL17 cells stimulated with 1 μM S1P (Upper) or 10 μg/mL anti-IgM F(ab’)2 (Lower) and immunoblotted for SHEP1 and CasL. (B) Lysates from splenic B cells from WT-CD19cre and SHEP1flox/flox-CD19cre mice immunoblotted for CasL, SHEP1, and actin. (C) B-cell lysates incubated with or without λ-protein phosphatase (λ-PP) and immunoblotted for CasL and actin. (D) Immunoprecipitated CasL from splenic B cells from WT-CD19cre and SHEP1flox/flox-CD19cre mice stimulated with 10 μg/mL anti-IgM F(ab’)2 and immunoblotted for phosphoserine and SHEP1. Total lysates were immunoblotted for actin. (E) Lysates from splenic B cells from WT-CD19cre and SHEP1flox/flox-CD19cre mice were stimulated with 1 μM S1P and immunoblotted for CasL and actin.