Figure 7. Transfection of pcDNA 3 constructs in LNCaP and DU-145 cell lines.

A) Western blot analysis of SHBG translation in LNCaP cells transfected with pcDNA 3-SHBG constructs with and without 5′UTR (exon 1A). Three independent experiments were performed. B) RPL and RPML were calculated. C) Western blot analysis of SHBG translation in DU-145 cells transfected with pcDNA 3-SHBG constructs with and without the 5′UTR (exon 1A). Three independent experiments were performed. D) RPL and RPML were calculated.