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. 2010 Nov 4;5(11):e13844. doi: 10.1371/journal.pone.0013844

Table 2. List of primers used for RT-PCR.

Primer localization Primer sequence Annealing temperature/cycles
Exon 2 upper Exon 5 lower 5′ TGTCATGACCTTTGACCTCACC 3′ 5′ TGAGATCTCGGCCTGTTTGTC 3′ 59°C/35
Exon 2 upper Exon 8 lower 5′ TGTCATGACCTTTGACCTCACC 3′ 5′ AGGGGGGTTCTTAGGTGGAGC 3′ 59°C/35
Exon 1A upper Exon 8 lower 5′ TTCAAAGGCTCCCCCGCAGTGC 3′5′ AGGGGGGTTCTTAGGTGGAGC 3′ 60°C/39
Exon 1B upper Exon 8 lower 5′ TGAAGAGCCTGAGAGAGCG 3′ 5′ AGGGGGGTTCTTAGGTGGAGC 3′ 59°C/39
S18 upper S18 lower 5′ GATGGGCGGGGGAAAAT 3′ 5′ CTTGTACTGGCGTGGATTCTGC 3′ 59–60°C/29