Table 2. List of primers used for RT-PCR.
Primer localization | Primer sequence | Annealing temperature/cycles |
Exon 2 upper Exon 5 lower | 5′ TGTCATGACCTTTGACCTCACC 3′ 5′ TGAGATCTCGGCCTGTTTGTC 3′ | 59°C/35 |
Exon 2 upper Exon 8 lower | 5′ TGTCATGACCTTTGACCTCACC 3′ 5′ AGGGGGGTTCTTAGGTGGAGC 3′ | 59°C/35 |
Exon 1A upper Exon 8 lower | 5′ TTCAAAGGCTCCCCCGCAGTGC 3′5′ AGGGGGGTTCTTAGGTGGAGC 3′ | 60°C/39 |
Exon 1B upper Exon 8 lower | 5′ TGAAGAGCCTGAGAGAGCG 3′ 5′ AGGGGGGTTCTTAGGTGGAGC 3′ | 59°C/39 |
S18 upper S18 lower | 5′ GATGGGCGGGGGAAAAT 3′ 5′ CTTGTACTGGCGTGGATTCTGC 3′ | 59–60°C/29 |