Table 1.
NTNH gene detection on C. botulinum and other clostridial strains
| BoNT subtype | strain | PCR(DNA)a | (culture supernatant)b | other clostridia | strain | PCR(DNA)a |
|---|---|---|---|---|---|---|
| A1 | Hall | + | + | C. absonum | ATCC 27555 | - |
| A1 | CDC 1757 | + | + | C. baratii e | ATCC 27638 | - |
| A1 | CDC 1744 | + | + | C. bifermentans | ATCC 638 | - |
| A2 | Kyoto-F | + | + | C. haemolyticum | ATCC 9650 | - |
| A2b | CDC 1436 | + | + | C. hastiforme | ATCC 25772 | - |
| A3 | Loch Maree | + | + | C. histolyticum | ATCC 19401 | - |
| B1 | Okra | + | + | C. novyi | ATCC 17861 | - |
| B1 | CDC 1656 | + | + | C. novyi | ATCC 19402 | - |
| B1 | CDC 1758 | + | + | C. novyi A | ATCC 19402 | - |
| B2 | 213B | + | + | C. novyi B | ATCC 2706 | - |
| B2 | CDC 1828 | + | + | C. perfringens A | ATCC 3624 | - |
| B4 (npB) | Eklund 17B | + | + | C. perfringens A | ATCC 12915 | - |
| Ba4 | CDC 657 | + | + | C. perfringens A | ATCC 12917 | - |
| Bf | An436 | + | + | C. perfringens A | ATCC 12918 | - |
| C | Stockholm | + | - | C. perfringens A | ATCC 12919 | - |
| C/D | 6813 | + | - | C. perfringens A | ATCC 13124 | - |
| D | ATCC 11873 | + | + | C. perfringens B | ATCC 3626 | - |
| D | 1873 | + | nd | C. perfringens D | ATCC 3629 | - |
| D/C | VPI 5995 | + | + | C. perfringens D | ATCC 3630 | - |
| E1 | Beluga | + | - | C. perfringens D | ATCC 3631 | - |
| E2 | CDC 5247 | + | nt | C. perfringens D | ATCC 12920 | - |
| E2 | CDC 5906 | + | nt | C. perfringens E | ATCC 27324 | - |
| E3 | Alaska E43 | + | + | C. ramosum | ATCC 25582 | - |
| E4 (It butyr)c | BL5262 | + | - | C. septicum | ATCC 12464 | - |
| F1 (prot) | Langeland | + | + | C. sordelli | ATCC 9714 | - |
| F2 (np) | Eklund 202F | + | - | C. sporogenes | ATCC 19404 | - |
| F3 (baratii)d | Orange | + | nt | C. sporogenes | ATCC3854 | - |
| G | 1354 | + | nd | C. subterminale | ATCC 25774 | - |
| C. tertium | ATCC 14573 | - | ||||
| C. tetani | ATCC 10799 | - | ||||
| C. tetani | ATCC19406 | - |
a +/- indicates presence/absence of 101 bp band on agarose gel. Samples are purified DNA from bacterial cultures as described in the Methods section.
b Samples originate from filtered culture supernatants containing crude toxin. +/- indicates presence/absence of 101 bp band on agarose gel. nd = not detected, nt = not tested.
c BoNT E-producing strain of C. butyricum isolated from an infant case in Italy.
d BoNT F-producing strain of C. baratii.
eNon-toxin producing strain of C, baratii.
Results from conventional PCR detection of NTNH. A (+/-) indicates presence/absence of 101 bp band by agarose gel, respectively. DNA results indicate PCR detection of NTNH in purified DNA from both C botulinum and other Clostridial strains. Culture supernatant results indicate amplification of DNA within crude culture supernatants. NT indicates samples that were not tested.