Table 2.
NTNH PCR detection in spiked healthy infant stool and a clinical infant botulism sample
| Strain (genomic copies) | NTNH Detection |
|---|---|
| BoNT A (104) | ++ |
| BoNT A (102) | ++ |
| BoNT A (10) | + |
| BoNT A (1) | + |
| BoNT B (104) | ++ |
| BoNT B (102) | + |
| BoNT B (10) | + |
| BoNT B (1) | - |
| BoNT C (104) | ++ |
| BoNT C (102) | + |
| BoNT C (10) | + |
| BoNT C (1) | - |
| BoNT D (104) | ++ |
| BoNT D (102) | + |
| BoNT D (10) | - |
| BoNT D (1) | - |
| BoNT E (104) | ++ |
| BoNT E (102) | + |
| BoNT E (10) | + |
| BoNT E (1) | - |
| BoNT F (104) | ++ |
| BoNT F (102) | ++ |
| BoNT F (10) | + |
| BoNT F (1) | - |
| BoNT G (104) | ++ |
| BoNT G (102) | ++ |
| BoNT G (10) | + |
| BoNT G (1) | - |
| Clinical stool sample | ++ |
++ Denotes strong PCR band
+ Denotes weak PCR band
- Denotes no PCR Band
Listed in this table are results from NTNH gene detection within spiked samples of a healthy infant stool sample as described in the materials and methods. Also included is the result from a confirmed case of infant botulism in California. (++) indicates a strong positive PCR product at the dilution tested, (+) is a weak positive PCR product, and (-) indicates no amplification detected.