. 2010 Oct 20;10:267. doi: 10.1186/1471-2180-10-267

Table 3.

Primer and probe sets for each serotype used in quantitative PCR

`Toxin Class`	`Sequence`	`Location on Toxin Gene(bp)`
`BoNT A Forward`	`TGGTTTTGAGGAGTCACTTGAA`	`582`
`BoNT A Reverse`	`TCATGTCCCCCAAATGTTCT`	`809`
`BoNT A Probe`	`TGCAGGCAAATTTGCTACAGATCCA`	`627`
`BoNT B Forward`	`CAAGAAAACAAAGGCGCAAG`	`619`
`BoNT B Reverse`	`CTGGGATCTTGYCCTCCAAA`	`833`
`BoNT B Probe`	`CGTGGATATTTTTCAGATCCAGCCTTG`	`652`
`BoNT C Forward`	`CAACTTTAATTATTCAGATCCTGTTGA`	`18`
`BoNT C Reverse`	`GGCTTGTAACTCGAGGAGGTT`	`199`
`BoNT C Probe`	`TGAGCCTGAAAAAGCCTTTCGCA`	`93`
`BoNT D Forward`	`CCATCATTTGAAGGGTTTGG`	`541`
`BoNT D Reverse`	`TGGGTCCATCTTGAGARAAA`	`791`
`BoNT D Probe`	`GATTCGTCCACAAGTTAGCGAGGGA`	`744`
`BoNT E Forward`	`ATAATGGGAGCAGAGCCTGA`	`448`
`BoNT E Reverse`	`CCCTTTAGCCCCATATAGTCC`	`678`
`BoNT E Probe`	`TGCCAAGCAATCACGGTTTTGG`	`515`
`BoNT F Forward`	`GTSAGACAATACCTCAAATATCAAATCG`	`1488`
`BoNT F Reverse`	`CTGGYACTTTTTGTGCATGT`	`1646`
`BoNT F Probe`	`TGCCAAGATATGATTCTAATGGAA`	`1551`
`BoNT G Forward`	`ATCCAACCTGGAGCTGAAGA`	`427`
`BoNT G Reverse`	`GCTGGATCTGCAAAATACGC`	`674`
`BoNT G Probe`	`TGGCCATTCCCCAATATCAGAAGG`	`534`

Y = C or T

R = A or G

S = G or C

Indicated in this table are the type specific primers and probes for each BoNT tested in this manuscript. Included are forward, reverse and probe sequences and their locations within the toxin gene. Bases indicated in bold represent degenerate bases: Y represents C or T; S represents C or G, and R represents A or G.