Table 5.
Detection limits of BoNT DNA in crude toxin supernatants
| Bot A | Bot B | Bot E | Bot F | |
|---|---|---|---|---|
| Crude Toxin 2 ng | LOD | |||
| Crude Toxin 200 pg | LOD | LOD | ||
| Crude Toxin 20 pg | 0.8 (LOD) | |||
| Crude Toxin 2 pg | ||||
| Crude Toxin 200 fg | 11.7 | 2.58 | ||
| Crude Toxin 20 fg | 29.2 |
LOD indicates the averaged limits of detection for that subtype in our mouse protection bioassay with identical serotypes used in toxin complex preparations.
Indicated in this table is the sensitivity of quantitative PCR for type-specific BoNT detection. Crude toxin supernatants with equivalent toxin protein amounts are listed. Values included in this table are the exact copy number of BoNT DNA detected in crude toxin preparations at the indicated amounts of protein. LOD indicates the averaged limits of detection for that subtype in our mouse protection bioassay with identical serotypes used in toxin complex preparations.