Figure 2.

Anti ED-B antibodies identified after three rounds of colony filter screening. (A) The Durapore filter membrane, where 103 bacterial clones were plated and which were rescued from the second round of iterative filter screening. (B) The capture filter membrane, coated with the recombinant protein 7B89, onto which antibodies secreted by the colonies grown on filter (A) could diffuse. The clones secreting antibodies binding to 7B89 were detected by enzymatic colorimetric reaction. Around 30% of the colonies in (A) result positive in (B).