Table 1. Sequence of the variable immunoglobulin domains of scFv(ME4C).

E1	V	Q	L	L	E	S	G	G	G10	L
V	Q	P	G	G	S	L	R	L20	S	C
A	A	S	G	F	T	F	S30	S	F	S
M	S	W	V	R	Q	A40	P	G	K	G
L	E	W	V	S	S50	I	S	G	S	S
G	T	T	Y	Y60	A	D	S	V	K	G
R	F	T	I70	S	R	D	N	S	K	N
T	L	Y80	L	Q	M	N	S	L	R	A
E	D90	T	A	V	Y	Y	C	A	K	Q99
K	S	A	P103	F	D	Y	W	G	Q	G110
T	L	V	T	V	S	S	G	D	G120	S
S	G	G	S	G	G	A	S	S130	S	E
L	T	Q	D	P	A	V	S140	V	A	L
G	Q	T	V	R	I	T150	C	Q	G	D
S	L	R	S	Y	Y160	A	S	W	Y	Q
Q	K	P	G	Q170	A	P	V	L	V	I
Y	G	K	N180	N	R	P	S	G	I	P
D	R	F190	S	G	S	S	S	G	N	T
A	S200	L	T	I	T	G	A	Q	A	E
D210	E	A	D	Y	Y	C	N	S	S	A220
P	V	S	N	R225	V	V	F	G	G230	G
T	K	L	T	V	L	G238

Primary sequence of the variable immunoglobulin domains of scFv(ME4C) (DDBJ/EMBL/GenBank accession no. AJ297960). Single amino acid codes are used according to standard IUPAC nomenclature. Amino acid positions in the CDR3, which are combinatorially mutated in the ETH-2 antibody library, are indicated in bold. A flexible linker connecting V_H and V_L domains is located between residues number 118 and 129.