Fig. 1.
Kaempferol attenuates UVB-induced COX-2 protein expression. (A) Chemical structure of kaempferol with its numbering scheme. (B) Kaempferol inhibits UVB-induced COX-2 protein expression in JB6 P+ cells. Cells were treated with various doses of kaempferol (0, 10, 20, or 40 μM) for 30 min, then stimulated with UVB (0.05 J/cm2) and harvested 4 h later. COX-2 and β-actin protein expression were determined by Western blotting using specific antibodies. Three separate experiments were performed with similar results and representative blots are shown. The asterisks (**) indicate a significant decrease in COX2 activity in groups treated with UVB and kaempferol compared with the group treated with UVB alone (p < 0.01).