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. Author manuscript; available in PMC: 2011 Dec 15.
Published in final edited form as: Biochem Pharmacol. 2010 Jul 1;80(12):2042–2049. doi: 10.1016/j.bcp.2010.06.042

Fig. 2.

Fig. 2

Kaempferol inhibits UVB-induced cox-2 and AP-1 promoter activity in JB6 P+ cells. (A and B) Kaempferol suppresses UVB-induced cox-2 and AP-1 promoter activity. For the luciferase assay, JB6 P+ cells stably transfected with a luciferase reporter plasmid bearing either cox-2 or AP-1 were cultured as described in Materials and Methods. Cells were then starved in 0.1% FBS-MEM and treated with various doses of kaempferol (0, 10, 20, or 40 μM) for 30 min. The cells were then subjected to UVB irradiation (0.05 J/cm2) and harvested 4 h later. Luciferase activity was assayed and cox-2 and AP-1 activities are expressed relative to control cells without UVB treatment. The values indicate means ± S.D. of cox-2- or AP-1-associated luciferase activity calculated from triplicate samples. The asterisk (**) indicates a significant decrease in luciferase activity in kaempferol treated cells compared to untreated control (p < 0.01).