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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Mol Biochem Parasitol. 2010 Oct 1;175(1):83–90. doi: 10.1016/j.molbiopara.2010.09.007

Fig. 2.

Fig. 2

Swelling rates of Xenopus oocytes expressing LmAQP1 with C-loop mutations. Oocytes were injected with water (control), wild-type, A163D, A163E, A163Q, A163S and A163T LmAQP1-cRNA. Swelling rates of oocytes were determined before and after 5 min incubation in ND96 medium with 0.3 mM HgCl2 as described in Materials and Methods. Inset: Western blot analysis of membrane fractions of Xenopus oocytes expressing wild type and altered LmAQP1.