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. Author manuscript; available in PMC: 2011 Nov 15.
Published in final edited form as: J Immunol. 2010 Oct 11;185(10):6214–6225. doi: 10.4049/jimmunol.0903843

Figure 10. Effect of KC disruption on cytokine/chemokine production and activation of NF-κB and MAPKs in AMs following Kp infection.

Figure 10

(A-C) Decreased production of MIP-2 in alveolar macrophages obtained from KC-/- mice to Kp challenge. Alveolar macrophages were stimulated with Kp at an MOI of 1 and supernatants collected at 18 h post infections were used to determine MIP-2, TNF-α, and KC levels. The data obtained from 2 independent experiments using 4 mice in total. (D) Reduced activation of NF-κB and MAPKs in alveolar macrophages obtained from KC-/- mice following Kp infection. Activation of IKK, NF-κB and MAPKs was detected at various time points following Kp stimulation (MOI of 1). The blot is a representative of three experiments with identical results. (E) Densitometric analysis of IKK, NF-κB and MAPK activation in BMMs up to 8 h following Kp stimulation with an MOI of 1. Data expressed as mean ± SE of three blots from three mice in each group from 2 independent experiments (p < 0.05).